Last position-specific scoring matrix computed, weighted observed percentages rounded down, information per position, and relative weight of gapless real matches to pseudocounts A R N D C Q E G H I L K M F P S T W Y V A R N D C Q E G H I L K M F P S T W Y V 1 A 4 -2 -2 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.46 0.04 2 D -2 -2 1 6 -4 -1 1 -2 -1 -4 -4 -1 -4 -4 -2 -1 -1 -5 -4 -4 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.07 3 C -1 -4 -3 -4 10 -3 -4 -3 -3 -2 -2 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.42 0.07 4 A 4 -2 -2 -2 -1 -1 -1 -1 -2 0 -1 -1 -1 -2 -1 1 0 -3 -2 2 77 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 23 0.36 0.07 5 K -1 2 0 -1 -3 1 0 -2 -1 -2 -2 5 -1 -3 -1 0 2 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 77 0 0 0 0 23 0 0 0 0.47 0.07 6 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -3 -1 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.16 0.07 7 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.64 0.07 8 I -1 -3 -4 -4 -1 -3 -3 -4 -4 5 1 -3 1 -1 -3 -3 -1 -3 -2 3 0 0 0 0 0 0 0 0 0 77 0 0 0 0 0 0 0 0 0 23 0.48 0.07 9 E -1 0 -1 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.85 0.07 10 F -2 -3 -3 -4 -3 -3 -3 -4 -1 -1 0 -3 0 6 -4 -3 -2 1 5 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 77 0 0 0 0 23 0 0.83 0.07 11 S 1 -1 0 -1 -1 0 -1 -1 -1 -2 -2 -1 -2 -3 -1 4 3 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 77 23 0 0 0 0.48 0.07 12 K -1 2 0 -1 -3 1 1 -2 -1 -3 -3 5 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0.65 0.08 13 Y -2 -2 -2 -4 -3 -2 -2 -3 2 -2 -1 -2 -1 3 -3 -2 -2 2 8 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 1.04 0.08 14 N -2 -1 7 1 -3 0 -1 -1 0 -4 -4 0 -3 -3 -2 0 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.87 0.08 15 E -1 -1 0 3 -4 1 5 0 -1 -4 -4 0 -3 -4 -2 0 -1 -4 -3 -3 0 0 0 24 0 0 69 7 0 0 0 0 0 0 0 0 0 0 0 0 0.68 0.08 16 D -2 -1 3 6 -4 0 2 -2 -1 -4 -4 -1 -3 -4 -2 0 -1 -4 -3 -3 0 0 18 67 0 0 14 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.08 17 N -2 -1 4 5 -4 0 1 -1 -1 -4 -4 -1 -3 -4 -2 0 -1 -5 -3 -3 0 0 35 65 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.82 0.08 18 T 0 -2 -1 -2 -1 -1 -1 -2 -2 1 -1 -1 -1 -2 -2 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 86 0 0 0 0.47 0.08 19 F -3 -3 -3 -3 -3 -3 -3 -3 3 -1 0 -3 0 6 -4 -2 -2 0 3 -1 0 0 0 0 0 0 0 0 14 0 0 0 0 86 0 0 0 0 0 0 0.75 0.08 20 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 2 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 86 0 0 0 0.54 0.08 21 V 0 -2 -3 -3 -1 -2 -2 -3 -3 2 1 -2 0 -1 -2 -2 0 -3 -1 4 1 1 1 1 0 1 1 1 0 1 1 1 0 1 1 1 1 0 0 87 0.34 0.07 22 K -1 2 0 -1 -4 1 2 -2 -1 -3 -3 5 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 14 0 0 0 0 86 0 0 0 0 0 0 0 0 0.59 0.08 23 V 0 -3 -3 -3 -1 -3 -3 1 -3 2 0 -3 0 -1 -3 -2 0 -3 -2 4 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 0 0 0 0 86 0.35 0.08 24 S 2 -2 0 3 -2 -1 0 3 -2 -3 -3 -1 -2 -3 -2 1 -1 -3 -3 -2 33 0 0 30 0 0 0 24 0 0 0 0 0 0 0 14 0 0 0 0 0.40 0.08 25 G 0 -2 0 3 -3 -2 -1 5 -2 -4 -4 -2 -3 -4 -2 0 -2 -3 -3 -4 0 0 0 26 0 0 0 74 0 0 0 0 0 0 0 0 0 0 0 0 0.84 0.06 26 R -1 3 0 -1 -3 1 0 -2 -1 -3 -3 5 -2 -3 -1 0 0 -3 -2 -2 0 13 0 0 0 0 0 0 0 0 0 80 0 0 0 0 7 0 0 0 0.54 0.06 27 E -1 0 0 1 -4 2 6 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.06 28 Y -2 -2 -3 -4 -2 -2 -3 -4 1 -1 1 -2 0 3 -3 -2 -2 2 7 -1 0 0 0 0 0 0 0 0 0 0 24 0 0 0 0 0 0 0 76 0 0.68 0.06 29 W -2 -3 -3 -4 -2 -2 -3 -3 -2 -2 -1 -3 -1 4 -3 -3 -2 10 2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 24 0 0 0 61 0 0 1.34 0.06 30 T 0 -1 0 2 -1 -1 -1 -2 -2 -1 -2 -1 -1 -3 -1 1 5 -3 -2 -1 0 0 0 15 0 0 0 0 0 0 0 0 0 0 0 0 85 0 0 0 0.48 0.06 31 N -1 0 5 1 -2 2 0 -1 0 -3 -3 0 -2 -3 -2 2 0 -3 -2 -3 0 0 57 0 0 15 0 0 0 0 0 0 0 0 0 28 0 0 0 0 0.51 0.06 32 R -1 6 0 -2 -3 1 0 -2 -1 -3 -3 2 -2 -3 -2 1 -1 -3 -2 -3 0 85 0 0 0 0 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0.69 0.06 33 W -3 -3 -4 -5 -2 -2 -3 -3 -3 -2 0 -3 -1 1 -4 -3 -3 11 2 -2 0 0 0 0 0 0 0 0 0 0 15 0 0 0 0 0 0 85 0 0 1.98 0.06 34 N -2 -1 6 1 -3 0 0 -1 0 -4 -4 0 -2 -3 -2 0 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.83 0.06 35 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.54 0.06 36 Q -1 1 0 -1 -3 6 2 -2 0 -3 -2 1 -1 -4 -2 0 -1 -2 -2 -3 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.75 0.06 37 P 0 -2 -1 -1 -3 -1 -1 -2 -2 -3 -3 -1 -3 -4 7 1 0 -4 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 76 24 0 0 0 0 1.47 0.06 38 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.54 0.06 39 L -2 -2 -4 -4 -2 -2 -3 -4 -3 1 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0.54 0.06 40 Q -1 0 -1 -1 -3 5 1 -2 0 -1 1 1 0 -2 -2 -1 -1 -2 -2 -1 0 0 0 0 0 76 0 0 0 0 24 0 0 0 0 0 0 0 0 0 0.45 0.06 41 S 1 -1 1 0 -1 0 0 0 -1 -3 -3 0 -2 -3 -1 4 1 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0.47 0.01 42 A 4 -2 -2 -2 0 -1 -1 0 -2 -1 -2 -1 -1 -2 -1 1 0 -3 -2 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.42 0.01 43 Q -1 1 0 0 -3 6 2 -2 0 -3 -2 1 0 -3 -1 0 -1 -2 -2 -2 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.67 0.01 44 L -2 -3 -4 -4 -1 -2 -3 -4 -3 2 4 -3 2 0 -3 -3 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 22 78 0 0 0 0 0 0 0 0 0 0.42 0.01 45 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.49 0.01 46 G 0 -3 0 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -3 -3 -3 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.06 0.01 47 M -1 -2 -2 -3 -2 0 -2 -3 -2 1 2 -1 6 0 -3 -2 -1 -2 -1 1 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0.36 0.01 48 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.49 0.01 49 V 0 -3 -3 -3 -1 -2 -3 -3 -3 3 1 -2 1 -1 -3 -2 0 -3 -1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0.36 0.01 50 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -3 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0.50 0.02 51 I -1 -3 -3 -3 -1 -3 -3 -4 -4 4 1 -3 1 0 -3 -2 -1 -3 -1 3 0 0 0 0 0 0 0 0 0 90 0 0 0 0 0 0 0 0 0 10 0.41 0.02 52 I -1 2 -1 -1 -3 1 0 -2 -1 0 -2 5 -1 -3 -2 -1 -1 -3 -2 -1 0 0 0 0 0 0 0 0 0 17 0 83 0 0 0 0 0 0 0 0 0.41 0.07 53 S 1 -1 0 -1 -1 0 -1 -1 -1 -2 -2 -1 -2 -3 -1 4 3 -3 -2 -2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 78 22 0 0 0 0.48 0.07 54 N 0 -1 5 1 -2 0 0 -1 0 -3 -3 0 -2 -3 -2 3 1 -4 -2 -3 0 0 56 0 0 0 0 0 0 0 0 0 0 0 0 44 0 0 0 0 0.55 0.07 55 T 2 -1 -1 -1 -1 -1 -1 -1 -2 -1 -2 -1 -1 -2 -1 1 5 -3 -2 0 22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 78 0 0 0 0.46 0.07 56 C -1 -4 -3 -4 9 -3 -4 -3 -3 -1 -2 -3 -2 -3 -3 -1 -1 -3 -3 -1 0 0 0 0 92 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.22 0.07 57 S 2 -1 0 0 -2 1 3 -1 4 -3 -3 0 -2 -3 -1 1 -1 -3 -1 -2 23 0 0 0 0 0 38 0 22 0 0 0 0 0 0 17 0 0 0 0 0.37 0.07 58 S 0 -1 3 0 -2 0 0 -1 -1 -3 -3 0 -2 -3 2 4 1 -3 -2 -2 0 0 22 0 0 0 0 0 0 0 0 0 0 0 10 68 0 0 0 0 0.47 0.07 59 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -2 -1 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.15 0.07 60 S 1 -1 0 -1 -1 -1 -1 3 -1 -3 -3 -1 -2 -3 -1 4 1 -3 -2 -2 0 0 0 0 0 0 0 22 0 0 0 0 0 0 0 78 0 0 0 0 0.49 0.07 61 G 0 -3 -1 -2 -3 -2 -2 6 -2 -4 -4 -2 -3 -4 -2 -1 -2 -3 -3 -4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 1.15 0.07 62 F -3 -3 -3 -4 -3 -4 -4 -4 -2 0 0 -4 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0.89 0.07 63 A 4 -2 -1 -2 -1 -1 -1 0 -2 -2 -2 -1 -1 -3 -1 2 0 -3 -2 -1 78 0 0 0 0 0 0 0 0 0 0 0 0 0 0 22 0 0 0 0 0.44 0.07 64 E -1 0 0 1 -4 3 5 -2 0 -4 -3 1 -2 -4 -1 0 -1 -3 -2 -3 0 0 0 0 0 10 90 0 0 0 0 0 0 0 0 0 0 0 0 0 0.79 0.07 65 V 0 -3 -3 -3 -1 -3 -3 0 -3 2 0 -3 0 -1 -3 -2 0 -3 -2 4 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 90 0.38 0.09 66 Q -1 0 -1 -1 -3 5 1 -2 2 1 -1 1 -1 -2 -2 -1 -1 -2 -1 -1 0 0 0 0 0 53 0 0 9 17 0 11 0 0 0 0 0 0 0 0 0.29 0.09 67 F -3 -3 -4 -4 -3 -4 -4 -4 -2 0 1 -3 0 7 -4 -3 -2 1 3 -1 0 0 0 0 0 0 0 0 0 0 9 0 0 91 0 0 0 0 0 0 0.84 0.09 68 N -2 -1 6 1 -3 0 0 -1 0 -4 -4 0 -2 -3 -2 1 0 -4 -2 -3 0 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.80 0.04 K Lambda Standard Ungapped 0.1273 0.3146 Standard Gapped 0.0410 0.2670 PSI Ungapped 0.1414 0.3185 PSI Gapped 0.0433 0.2670