This folder includes: 

1) normalize.pl (A perl program to convert absolute ASA to relative ASA and vice versa)
2) HOA.txt file (This file contains the HOA data of all tripeptide. Can be edited to replace values)
3) ASA_file.txt (example file format for absolute ASA)
4) RSA_file.txt (example file format for relative ASA)


(I) Calculating RSA by using HOA (Highest observed ASA)

	Usage: perl normalize.pl -R -i ASA_file.txt -H HOA.txt # To calculate relative ASA using HOA values. 
        		     OR
      	       perl normalize.pl -A -i RSA_file.txt -H HOA.txt # To calculate absolute ASA using HOA values.

	And to save the output of the program:

	Usage:  perl normalize.pl -R -i ASA_file.txt -H HOA.txt > rsa.txt 
                         OR
       		perl normalize.pl -A -i RSA_file.txt -H HOA.txt > abs_ASA.txt  


(II) Calculating RSA using Ala-X-Ala (Traditional ESA normalization).

        Usage: perl normalize.pl -R -i ASA_file.txt # To calculate relative ASA using Ala-X-Ala.
                             OR
               perl normalize.pl -A -i RSA_file.txt # To calculate absolute ASA using Ala-X-Ala.

        And to save the output of the program:

        Usage:  perl normalize.pl -R -i ASA_file.txt  > rsa.txt 
                         OR
                perl normalize.pl -A -i RSA_file.txt  > abs_ASA.txt  

